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Reporter fluorescence changes in response to topoisomerase inhibitors. Flow cytometry histograms comparing green fluorescence intensities in 17-week-old reporter organoids exposed to A) 1 µM topotecan, B) 1 µM <t>irinotecan,</t> and C) 0.2% DMSO in water (vehicle control). The parental and reporter iPSCs were used to determine the GF +/-gate. a.u. - arbitrary units.
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Reporter fluorescence changes in response to topoisomerase inhibitors. Flow cytometry histograms comparing green fluorescence intensities in 17-week-old reporter organoids exposed to A) 1 µM topotecan, B) 1 µM <t>irinotecan,</t> and C) 0.2% DMSO in water (vehicle control). The parental and reporter iPSCs were used to determine the GF +/-gate. a.u. - arbitrary units.
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Reporter fluorescence changes in response to topoisomerase inhibitors. Flow cytometry histograms comparing green fluorescence intensities in 17-week-old reporter organoids exposed to A) 1 µM topotecan, B) 1 µM <t>irinotecan,</t> and C) 0.2% DMSO in water (vehicle control). The parental and reporter iPSCs were used to determine the GF +/-gate. a.u. - arbitrary units.
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Reporter fluorescence changes in response to topoisomerase inhibitors. Flow cytometry histograms comparing green fluorescence intensities in 17-week-old reporter organoids exposed to A) 1 µM topotecan, B) 1 µM <t>irinotecan,</t> and C) 0.2% DMSO in water (vehicle control). The parental and reporter iPSCs were used to determine the GF +/-gate. a.u. - arbitrary units.
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Reporter fluorescence changes in response to topoisomerase inhibitors. Flow cytometry histograms comparing green fluorescence intensities in 17-week-old reporter organoids exposed to A) 1 µM topotecan, B) 1 µM <t>irinotecan,</t> and C) 0.2% DMSO in water (vehicle control). The parental and reporter iPSCs were used to determine the GF +/-gate. a.u. - arbitrary units.
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Reporter fluorescence changes in response to topoisomerase inhibitors. Flow cytometry histograms comparing green fluorescence intensities in 17-week-old reporter organoids exposed to A) 1 µM topotecan, B) 1 µM irinotecan, and C) 0.2% DMSO in water (vehicle control). The parental and reporter iPSCs were used to determine the GF +/-gate. a.u. - arbitrary units.

Journal: bioRxiv

Article Title: A Human Angelman Syndrome Class II Pluripotent Stem Cell line with Fluorescent Paternal UBE3A Reporter

doi: 10.1101/2025.07.12.664539

Figure Lengend Snippet: Reporter fluorescence changes in response to topoisomerase inhibitors. Flow cytometry histograms comparing green fluorescence intensities in 17-week-old reporter organoids exposed to A) 1 µM topotecan, B) 1 µM irinotecan, and C) 0.2% DMSO in water (vehicle control). The parental and reporter iPSCs were used to determine the GF +/-gate. a.u. - arbitrary units.

Article Snippet: Topotecan hydrochloride and irinotecan hydrochloride (both from Molcan Corporation) were directly added to 17-week-old AS reporter organoids at a final concentration of 1μM in culture.

Techniques: Fluorescence, Flow Cytometry, Control